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Fig. 5 | BMC Complementary Medicine and Therapies

Fig. 5

From: Administration of mulberry leaves maintains pancreatic β-cell mass in obese/type 2 diabetes mellitus mouse model

Fig. 5

The effect of an ML-diet on the proliferation of β-cells in db/db mice. a Representative fluorescence image of a pancreas from a 10-w old db/db mouse stained with anti-PCNA antibodies (green), anti-insulin antibodies (red), and DAPI (blue). White triangles indicate PCNA-positive cells. Scale bar: 50 μm. The ratio of the number of PCNA-positive β-cells to total β-cell number is shown in the graph (right panel). b A representative fluorescence image of a pancreas from a 15-w old db/db mouse stained with anti-PDX1 antibodies (red), anti-insulin antibodies (green) and DAPI (blue). The ratio of the number of PDX1-positive cells to the total β-cell number is shown in the graph (right panel). c Expression levels of Pdx1 mRNA in the pancreas of a 15-w old db/db mouse were analyzed via RT-qPCR. The relative Pdx1 mRNA level of the ML group to the control group is shown. CL, control-diet group; ML, ML-diet group, n = 6. All data are expressed as mean ± SEM. **, P < 0.01 versus control

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