Skip to main content
Fig. 4 | BMC Complementary Medicine and Therapies

Fig. 4

From: Administration of mulberry leaves maintains pancreatic β-cell mass in obese/type 2 diabetes mellitus mouse model

Fig. 4

The effect of an ML-diet on ER stress in β-cells of db/db mice. a mRNA expression of ER stress markers in pancreases of 10-w old mice and 15-w old mice were analyzed via RT-qPCR. The results are shown as relative mRNA levels of the ML-diet group to the control-diet group. b Immunohistological analyses of CHOP and ATF4. Pancreases of 10-w old and 15-w old mice were stained with anti-CHOP or anti-ATF4 antibodies (green), anti-insulin antibodies (red), and DAPI (blue), observed under a confocal fluorescence microscope. 1–4, pancreas from a 10-w old mouse; 5–8, pancreas from a 15-w old mouse; 1, 2, 5, 6; stained with anti-CHOP antibodies; 3, 4, 7, 8; stained with anti-ATF4 antibodies. The ratio of the number of CHOP or ATF4-positive β-cells to total β-cells are shown in the lower panel; n = 6. CL and white bar, control group; ML and black bar, ML-diet group. All data are expressed as mean ± SEM. *, P < 0.05; **, P < 0.01

Back to article page