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Table 1 Nucleotide sequences used for RT-PCR detection of cytokine mRNA expression

From: Ethyl acetate extract of Kaempferia parviflora inhibits Helicobacter pylori-associated mammalian cell inflammation by regulating proinflammatory cytokine expression and leukocyte chemotaxis

Gene

Primers

Product size

IL-8

Forward primer 5′-TCC AAA CCT TTC CAC CCC AA-3′

Reverse primer 5′-ACT TCT CCA CAA CCC TCT GC-3′

153 bp

GAPDH

Forward primer 5’-CTG ACT TCA ACA GCG ACA CC-3′

Reverse primer 5′-GTG GTC CAG GGG TCT TAC TC-3′

172 bp