Fig. 1

Effect of EECM on osteoblast differentiation of MC3T3-E1 cells and osteoclast differentiation of Raw264.7 cells. a Cell viability after 48 h of EECM treatment in MC3T3-E1 osteoblasts. b ALP activity in MC3T3-E1 after cells were differentiated for 7 days in differentiation medium with EECM. c TRAP activity in RAW264.7 cells after culturing for 7 days with RANKL (50 ng/mL). d ARS staining in MC3T3-E1 cells and (e) ARS-stained cells. f Western blot analysis and (g-i) real-time PCR analysis of osteoblastic markers in MC3T3E1 cells. *p < 0.05, **p < 0.01, ***p < 0.001, compared to control and differentiated cells