Fig. 3
A. Representative images of the immunocytochemical analysis of the chosen autophagy and apoptosis-related proteins in SW480 cell line in four conditions: control, incubation with 6-shogaol only, incubation with 5-FU only and treatment with 6-shogaol + 5-FU together. B. Results of immunocytochemical analysis of SW480 cell line and the chosen autophagy and apoptosis-related protein expression calculated by immunoreactivity score. Abbreviations: 0- no staining, 1- weak staining, 2-moderate staining, 3-strong staining. Control- cells cultured in complete culture medium for 48 h, 6-Shogaol- cells incubated with the compound in dose 25 μM, FU- cells treated with the drug in concentration 125 μM, 6-Shogaol + FU- cells treated with the compound and the drug together. Scale bar = 50 μm.