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Fig. 9 | BMC Complementary Medicine and Therapies

Fig. 9

From: Investigation of cardiovascular protective effect of Shenmai injection by network pharmacology and pharmacological evaluation

Fig. 9

Effects of SMI on DOX-induced apoptosis in H9c2 cells. Cells were pretreated with SMI (0.25, 0.5%) for 24 h, then stimulated with DOX (0.5 μM) for 24 h. a Damaged nucleus was examined by Hoechst 33342 staining. Damaged nuclei are marked with white arrows. b Cell apoptosis was assessed by TUNEL assay. c Protein levels of cleaved caspase-9, caspase-9, cleaved caspase-3 and caspase-3 were measured by Western blot. **P < 0.01 vs. Control; #P < 0.05 vs. DOX; ##P < 0.01 vs. DOX

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