Fig. 10

Western blot analysis of PI3K, Akt and p-Akt expression in H9c2 cells. a Cells were pretreated with SMI (0.25, 0.5%) for 24 h, then stimulated with DOX (0.5 μM) for 24 h. b Cells were pretreated with SMI (0.5%) for 24 h, LY294002 (10 μM) for 1 h, then stimulated with DOX (0.5 μM) for 24 h. PI3K, Akt and p-Akt expression were assessed by Western blot. *P < 0.05 vs. Control; **P < 0.01 vs. Control; ^#P < 0.05 vs. DOX; ^$P < 0.05 DOX + SMI vs. DOX + SMI + LY294002; ^$$P < 0.01 DOX + SMI vs. DOX + SMI + LY294002