Fig. 7

Immunofluorescence detection of the effects of Heshouwuyin on the protein expression of Cyt c, Caspase-9 and Caspase-3 in spermatogenic cells (bar = 50 μm): a: Immunofluorescence staining results of Cyt c, Caspase-9 and Caspase-3; b, c, d: Statistical analysis of average optical density of Cyt c, Caspase-9, and Caspase-3. ^ap < 0.05 vs. AG; ^bp < 0.05 vs. Apaf-1-siRNA-transfected group (n = 3)