Fig. 4

Screening for the optimal time and optimal sequence of siRNA-Apaf-1 (bar =50 μm) a, b, c: Transfection was performed at 24 h, 48 h, and 72 h; d: mean optical density statistics of Cy3 in spermatogenic cells transfected at 24 h, 48 h and 72 h (^ap < 0.05 vs 24 h; ^bp < 0.05 vs 48 h); e: Apaf-1 mRNA expression level of spermatogenic cells; f, g: Apaf-1 protein expression in spermatogenic cells (^ap < 0.05 vs. NCG; ^bp < 0.05 vs. NC-siRNA-transfected group; ^cp < 0.05 vs. siRNA3-transfected group) (n = 3)