Fig. 4

Immunoblot analysis for p-AMPK, GPR43 in liver (a) and muscle (b) after treatment with MET or PPM for 12 weeks. GAPDH was used as an internal control. In the histogram, values are presented as the mean ± S.D. (n = 10 rats per group). After treatment with MET and PPM, p-AMPK expression in liver and muscle increased. Increased GPR43 expression in liver and decreased GPR43 expression in muscle when compared to controls. ^*P < 0.05, ^**P < 0.01, and ^***P < 0.001 compared to the normal control group, ^#P < 0.05, ^##P < 0.01, and ^###P < 0.001 compared to the model control group