Fig. 7
From: Role of limonin in anticancer effects of Evodia rutaecarpa on ovarian cancer cells
Effects of limonin on cisplatin resistance of ovarian cancer cells. (a) Viability of normal and cisplatin-resistant (CisR) SKOV-3 cells treated with cisplatin (0.01–100 mM) for 72 h. (b) IC50 values of cisplatin against CisR and age-matched parental SKOV-3 cells. (c) Viability of CisR cells treated with limonin (1, 2, 5, and 10 μM). (d) Caspase-3 activity and (e) immunoblotting analysis of expression levels of proteins involved in the apoptotic pathway in CisR SKOV-3 cells exposed to limonin (1, 2, 5, and 10 μM) alone, limonin plus PTF-α (10 μM), or DMSO (control) for 24 h. α-Tubulin was used as a loading control. Data are expressed as the mean ± SEM. *P < 0.05, **P < 0.01