Fig. 6

Effects of the p53 inhibitor PFT-α on limonin-induced apoptosis in the SKOV-3 ovarian cancer cell line. (a) Immunoblotting analysis of expression levels of proteins involved in the apoptotic pathway in cells treated with limonin alone (10 μM), limonin plus PFT-α (10 and 50 μM), and DMSO (control) for 24 h. (b–g) Quantification of p53 (b), Bax (c), Bcl-2 (d), survivin (e), cleaved caspase-3 (f), and cleaved PARP1 (g) levels (mean ± SEM) in (a). α-Tubulin was used as a loading control. *P < 0.05, **P < 0.01