Fig. 4
From: Role of limonin in anticancer effects of Evodia rutaecarpa on ovarian cancer cells
Effects of limonin on subcellular localization of the p53 protein. (a) Immunoblotting analysis of p65 and p53 protein levels in subcellular fractions of cells treated with increasing concentrations of limonin or with DMSO (control). α-Tubulin and histone H3 were used as loading controls for the cytosol and nuclear fractions, respectively. (b, c) Z-stack images of subcellular localization of p65 (b) and p53 (c) in SKOV-3 cells. Scale bar = 5 μm. The arrowheads indicate nuclear localization