Mitochondrial dependent pathway is involved in the protective effects of carboxymethylated chitosan on nitric oxide-induced apoptosis in chondrocytes

Table 1 Primer sequences of target genes

Gene	Primer	Sequence	Product size (bp)
Bcl-2	Forward	TACGAGTGGGATACTGGAGA	165
Bcl-2	Reverse	TCAGGCTGGAAGGAGAAGT	165
Bax	Forward	GTTACAGGGTTTCATCCAGG	175
Bax	Reverse	CGTGTCCACGTCAGCAATAC	175
Cyt c	Forward	AAATGGGTGATGTTGAAGCT	139
Cyt c	Reverse	TTGGTCCAGTCTTATGCGGCT	139
Caspase-3	Forward	CTGGACTGCGGTATTGAGTG	156
Caspase-3	Reverse	GGGTGCGGTAGAGTAAGCG	156
GAPDH	Forward	TGTCTCCTGCGACTTCAACAG	256
GAPDH	Reverse	GAGGCCATGTAGGCCATGAG	256

ISSN: 2662-7671