Fig. 5

Effect of CaMKKβ inhibitor STO-609 on AA-24-a-induced phosphorylation of AMPK, ACC, AS160 and p38 MAPK. Myotubes were incubated in the presence or absence of STO-609 (15 μM) for 1 h followed by exposure to AA-24-a (40 μM) for 6 h. Western blotting of whole cell lysates to detect phosphorylation of AMPK, ACC, AS160 and p38 MAPK. Western blotting (a) and quantification (b, c) of phospho-AMPK and phospho-ACC. Western blotting (d) and quantification (e, f) of phospho-AS160 and phospho-p38 MAPK. Values are expressed as means ± SD (n = 3 or 4). ^* P < 0.05, ^** P < 0.01, vs. control group; ^# P < 0.05, ^## P < 0.01, vs. AA-24-a group