Fig. 3
From: Alisol A-24-acetate promotes glucose uptake via activation of AMPK in C2C12 myotubes
Effects of AMPK inhibitor compound C on AA-24-a-stimulated glucose uptake and GLUT4 translocation. C2C12 myotubes were incubated in the presence or absence of compound C (15 μM) for 1 h followed by exposure to AA-24-a for 12 h. (a) CCK-8 assay of cell viability. b 2-NBDG assay of glucose uptake. Western blotting (c) and quantification (d) of GLUT4 in cell membrane proteins. Values are expressed as means ± SD (n = 3). * P < 0.05, ** P < 0.01, vs. control group; # P < 0.05, ## P < 0.01, vs. AA-24-a group