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Table 1 Identification of active components in Dihuang and Shu Dihuang sample by HPLC-FTMS

From: “Quantity-effect” research strategy for comparison of antioxidant activity and quality of Rehmanniae Radix and Rehmannia Radix Praeparata by on-line HPLC-UV-ABTS assay

Peak

RT

[M-H]-

Chemical formula

MS2

Identification

1

6.22

397.0238

C15H23O10Cl

379.1123,276.9031,252.2112

glutinoside

2

8.55

495.1254

C26H23O10

477.1181,379.1190, 217.0684, 199.0581

unknown

3

14.37

731.2100

C29H33O14N9

505.1492, 323.0935, 263.0734,221.0633

unknown

4

17.25

373.1073

C16H22O10

283.0792,211.0580,167.0687,123.0431

geniposidic acid

5

18.61

375.1246

C16H24O10

315.1030,255.0831,213.0735,169.0843, 125.0586

8-epiloganic acid

6

19.60

389.1168

C17H26O10

183.0634,165.0531,139.0377

loganin

7

20.55

461.1592

C20H30O12

315.1039,297.0937,161.0429,135.0429

decaffeoylacteoside

8

21.94

375.1246

C16H24O10

315.1021,213.0738,169.0843,151.0740

mussarnosidic acid

9

29.57

343.0262

C15H20O9

299.1118,284.0858,197.0424,182.0192

syringic acid-4-O-α-L-rhamnoside

10

32.87

475.1747

C21H32O12

329.1194,311.1093,143.0583,161.0429

darendoside B

11

41.02

785.2394

C35H45O20

623.2108,461.1603,161.0218

echinacoside

12

43.60

785.2394

C35H45O20

623.2108,461.1603,161.0218

isomer of echinacoside

13

45.74

799.2570

C36H48O20

623.2102,605.1965,461.1597,315.1040

jionoside A1/A2

14

50.14

623.1865

C29H36O15

477.1354,461.1599,443.1508,315.1039,179.0321,161.0218

verbascoside

15

50.98

813.2729

C37H50O20

637.2254,491.1701,193.0477,175.0373

jionoside B1/B2

16

52.17

623.1865

C29H36O15

461.1600,315.1040,251.0525,179.0322,161.0218

isoverbascoside

17

54.61

637.2048

C30H38O15

491.1492,475.1773,461.4599,443.1497

jionoside D/leucosceptoside A

18

59.89

651.2176

C31H40O15

505.1632 475.1754 193.0477

martynoside/isomer

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