Table 1 Sequences of primers used in the RT-PCR analysis
Targets | Direction | Primer sequences |
|---|---|---|
iNOS | F | 5’-CTC AGC CCA ACA ATA CAA G-3’ |
R | 5’-CTA CAG TTC CGA GCG TCA-3’ | |
IL-6 | F | 5’-CTG CAA GAG ACT TCC ATC CAG-3’ |
R | 5’-AGT GGT ATA GAC AGG TCT GTT GG-3’ | |
MCP-1 | F | 5’-GCC CCA CTC ACC TGC TGC TAC T-3’ |
R | 5’-CCT GCT GCT GGT GAT CCT CTT GT-3’ | |
IL-1β | F | 5’-GCA ACT GTT CCT GAA CTC AAC T-3’ |
R | 5’-ATC TTT TGG GGT CCG TCA ACT-3’ | |
TNF-α | F | 5’- GCC TAT GTC TCA GCC TCT T-3’ |
R | 5’- GGT TGA CTT TCT CCT GGT AT-3’ | |
GAPDH | F | 5’-GGT TGT CTC CTG CGA CTT CA-3’ |
R | 5’-TGG TCC AGG GTT TCT TAC TCC-3’ |