Fig. 5

Effects of Cl-EE on LPS-induced activation of NF-κB signaling pathway. a Effect of Cl-EE on LPS-induced NF-κB luciferase action. RAW264.7 cells transfected with the luciferase reporter pNFκB-TA-luc were pretreated with Cl-EE at the indicated concentrations for 1 h and then exposed to LPS (10 ng/mL) for 4 h. NF-κB activity was detected by luciferase assay system. b-d Effects of Cl-EE on cytosol IκBα, cytosol p-IκBα and the nuclear translocation of NF-κB p65 in LPS-activated RAW264.7 cells. The cells were pretreated with Cl-EE at the indicated concentrations for 1 h and then were exposed to LPS (10 ng/mL) for 30 min. Cytosolic and nuclear proteins were extracted for Western blot analysis. ^##P < 0.01 versus NC group