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Fig. 2 | BMC Complementary and Alternative Medicine

Fig. 2

From: Helminthostachys zeylanica alleviates hepatic steatosis and insulin resistance in diet-induced obese mice

Fig. 2

Inhibition by HZ extract of palmitate-induced lipid accumulation in a human fatty liver cell model. a Quantitative analysis of lipid deposition in the Oil-Red O stained HuS-E/2 cells. b Images of the Oil-Red O stained HuS-E/2 cells were captured using a microscope at 400X original magnification. c Western blotting for phosphorylation of AMPK at Thr172 and ACC at Ser-79, total AMPK, ACC and tubulin. d Western blotting for SREBP-1c, CPT1, and tubulin. Tubulin served as a loading control. Quantitative analysis with Multi Gauge V3.0 is shown. e The levels of fatty acid β oxidation-related genes, PPARα and PPARδ. f The levels of fatty acid synthesis-related genes, SREBP-1c and PPARγ. The data represent the mean ± SEM for three independent experiments. ND vs. palmitate: *p < 0.05; **p < 0.01; ***p < 0.001. palmitate vs. HZ extract: #p < 0.05; ##p < 0.01; ###p < 0.001

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