Fig. 4
From: The pro-apoptotic effect of a Terpene-rich Annona cherimola leaf extract on leukemic cell lines
The quantitative assessment of apoptosis induced by AELE using Annexin V/PI. Monomac-1 (a) and KG-1 (b) were treated with the two concentrations of AELE within which the IC50 falls (173 and 346 μg/mL), followed by staining with Annexin V/PI, and analysis using flow cytometry. A shift from double-negative staining, to Annexin V-positive and PI-negative staining, an early apoptotic marker, upon treatment with AELE was observed. A slight increase in double positive stained cells was also observed