MEDD effects on STAT1 and STAT3 in IMQ-induced psoriasis
The dorsal skin of mice was treated with Vaseline, IMQ, IMQ and Vh or MEDD for 7 days, and the immune cell population was analyzed by Western blotting in the skin lysates the next day. (left) Antibodies specific for pSTAT1, STAT1, pSTAT3, STAT3, and β-actin were used. Western blot performed on four samples per group. Among them, two samples per group were selected and are presented as representative images. (right) Densitometry quantification of proteins. Arrow indicates 86 (upper) and 79 (bottom) kDa size. These results represent mean ± S.D. of four independent experiments. Statistical analysis was performed using Student’s t-test. * p < 0.05, compared with mice in the IMQ- and vehicle-treated groups; #, p < 0.05 compared with Vaseline control mice; IMQ, imiquimod; Vh, Vehicle; MEDD, methanol extract of Dictamnus dasycarpus root bark.