Fig. 4From: Anti-adipogenic and anti-obesity activities of purpurin in 3T3-L1 preadipocyte cells and in mice fed a high-fat dietEffects of purpurin on intracellular ROS levels, mitochondrial membrane potential, and intracellular ATP levels in 3T3-L1 cells treated with MDI for 48 h: a cells were labeled with DCF-DA for cellular ROS detection, and JC-1 for the detection of mitochondrial membrane potential; b cells were lysed, centrifuged and the supernatant mixed with luciferase/luciferin reagent, after which free ATP was detected with a luminometer. Data are expressed as the mean ± SD of triplicate experiments. Bars sharing a common letter are not significantly different at p < 0.05Back to article page