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Fig. 3 | BMC Complementary and Alternative Medicine

Fig. 3

From: 1α, 25-dihydroxy Vitamin D3 containing fractions of Catharanthus roseus leaf aqueous extract inhibit preadipocyte differentiation and induce lipolysis in 3T3-L1 cells

Fig. 3

CRACE decreased lipid accumulation and lipid droplet size in mature adipocytes. 3T3-L1 cells were differentiated to mature adipocytes followed by treatment with CRACE (500 μg/mL equivalent) for 4 days. Lipid content was estimated by ORO staining. a. Top panel represents design of the experiment. Micrographs represent ORO stained preadipocytes and CRACE treated and untreated mature adipocytes at doses of 300 and 500 μg/mL. Scale bars: 100 μm. b and c. Quantification of lipid in cells shown in panel a by ORO elution (b) and by measuring total ORO fluorescence per micrograph frames (c). Bars represent mean ± SEM. For panel b, n = 3. For panel c, n = 10 image frames. Unpaired t-test was performed to study the statistical significance. * p < 0.05 for untreated vs. CRACE treated. d-f. CRACE decreased size of lipid droplets in mature adipocytes. d. High magnification fluorescent micrographs of ORO stained untreated and CRACE treated mature adipocytes are shown in panel a. Scale bars: 10 μm. e. Population distribution of small to large size lipid droplets in the cells shown in panel d. Bars represent mean ± SEM. n = 5 image frames. * p < 0.05 for untreated Vs CRACE treated. One-way ANOVA followed by Bonferroni post-test was performed to evaluate the statistical significance. f. Average lipid droplet size calculated from the data represented in panel e. Box and whisker (minimum to maximum) representation of lipid droplet sizes in each group. Unpaired t-test was performed to study the statistical significance. * p < 0.05 for untreated Vs CRACE treated

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