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Fig. 5 | BMC Complementary and Alternative Medicine

Fig. 5

From: Alcohol extract from Vernonia anthelmintica willd (L.) seed counteracts stress-induced murine hair follicle growth inhibition

Fig. 5

Effects of AVE on peptidergic innervation in murine dorsal skin. Histochemical detection reveals the location and expressions of SP+ and CGRP+ nerve fibers (red staining, pointed out by arrows) by immunofluorescence (a), and the number of SP+ and CGRP+ nerve fibers per visual field in the skin from four mice groups were measured (b). In the immunofluorescence micrographs, nerve fibers are labeled in red, and cell nuclei are labeled blue with DAPI. The number of nerve fibers was quantified at least 10 visual fields per mouse, 3–5 mice each group. Results shown are mean ± S.E.M. ***p < 0.001 compared with control; ###p < 0.001 compared with RS group. Abbreviations: e, epidermis; d, dermis; hf, hair follicle. Magnification: 200 ×

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