Fig. 4

Effects of AVE on melanogenic proteins in murine dorsal skin. a Representative western blot analysis of the expressions of TYR, TRP-1, TRP-2 and MITF. b Quantitation of western blot analysis for TYR, TRP-1, TRP-2 and MITF. Equal protein loadings were confirmed using β-actin antibody. Total skin proteins (30 μg) were subjected to 10% SDS-PAGE. Densitometric scanning of band intensities obtained from three separate experiments by the Quantity One 1D analysis software program was used to quantify the change of proteins expressions (control value taken as onefold in each case). The data showed the mean ± S.E.M of three experiments. * p < 0.05 compared with CTRL group; # p < 0.05, ## p < 0.01 compared with RS group