Fig. 1

Effects of ChondroT and its constituent herbs on RANKL-induced osteoclast differentiation in BMDMs. a TRAP-positive stained cells were stained with TRAP staining kit. b TRAP-positive stained MNCs with more than three nuclei were counted as osteoclasts. c TRAP activity in osteoclast culture supernatant was determined at 540 nm. ^**P < 0.01; ^***P < 0.001 versus RANKL group; ⁺⁺⁺P < 0.001 versus control group; ^#P < 0.05; ^##P < 0.01; ^###P < 0.001 versus ChondroT group. d BMDMs pretreated with ChondroT or its constituent herbs for 72 h were treated with MTT solution for 4 h, and then were dissolved in DMSO. Absorbance was read at 570 nm. ^**P < 0.01; ^***P < 0.001 versus control group. Abbreviations: c1: 0.1 mg/ mL; c2: 0.3 mg/ mL; c3: 0.5 mg/mL