Fig. 5

AS-IV reversed HG-induced eNOS impairment in HRGECs. a The NO producing ability in HRGECs after high glucose treatment was assessed by Nitric Oxide (NO) assay kit. All results were expressed as mean ± SD (n = 3). b The expressions of eNOS, p-eNOS (Ser1177) and p-eNOS (Thr495) after high glucose treatment in HRGECs were detected by western blot. ****p < 0.0001, ***p < 0.001 vs. control; ###p < 0.001, ##p < 0.01 vs. HG; %%p < 0.01 vs HG + AS-IV