Fig. 5
Effect of PGE on splenic NK cell and CTL cytotoxicity in splenocytes. Splenocytes were prepared and assayed for NK cell (a) and CTL (b) activity using the WST-1 assay. Splenocytes were co-cultured with target cells (AR42J or HL-60) for NK cell or CTL activity, respectively, in 96-well plates, followed by treatment with PGE (0, 5, 10, 30, 50, 100, and 300 μg/mL) and incubated for 24 h in a 5% CO2 incubator with an effector to target cell ratio of 25:1. NK cell and CTL activities were calculated as the survival rate of AR42J or HL-60 cells compared to that of control cells. Bars labeled with different superscripts have significantly different values (P < 0.05). Data are presented as the means ± standard error (n = 3)