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Table 4 Determination of the total viable bacterial and fungal counts (CFU/g) in herbal medicine samples

From: Microbial contamination in herbal medicines: a serious health hazard to elderly consumers

Herbal medicine

Acceptable limits (CFU/g ≤ 105)a count/g or mL

Unacceptable limits (CFU/g > 105)a count/g or mL

Total viable aerobic bacterial count

Total viable fungal count

Total viable aerobic bacterial count

Total viable fungal count

Preparation

Use

Forms

Samples % (N)

% (N)

% (N)

% (N)

% (N)

Homemade herbal medicines

Oral

Solid

5.3 (7)

1.5 (2 ± 0.8)

1.5 (2 ± 1.1)

2.3 (3 ± 0.5)

3.0 (4 ± 1.8)

Semisolid

1.5 (2)

0 (0 ± 0.2)

0 (0)

0.8 (1 ± 0.2)

0.8 (1 ± 1.0)

Liquid

23.5 (31)

10.6 (14 ± 1.2)

3.0 (4 ± 1.1)

10.6 (14 ± 1.5)

3.0 (4 ± 2.3)

Topical

Solid

3.0 (4)

0.8 (1 ± 0.3)

0 (0)

1.5 (2 ± 0.4)

1.5 (2 ± 2.0)

Semisolid

2.3 (3)

0 (0)

0.8 (1 ± 0.2)

0 (0)

0.8 (1 ± 0.8)

Liquid

3.8 (5)

0 (0)

1.5 (2 ± 1.0)

1.5 (2 ± 0.5)

1.5 (2 ± 1.4)

Total

39.4 (52)

12.9 (17 ± 1.8)

6.8 (9 ± 0.9)

16.7 (22 ± 2.3)

10.6 (14 ± 1.9)

Commercial herbal medicines

Oral

Solid

7.6 (10)

0.8 (1 ± 0.5)

1.5 (2 ± 0.5)

3.0 (4 ± 0.5)

3.0 (4 ± 1.3)

Semisolid

0 (0)

0 (0)

0 (0)

0 (0)

0 (0)

Liquid

36.4 (48)

6.0 (8 ± 1.1)

3.0 (4 ± 1.2)

8.3 (11 ± 1.8)

8.3 (11 ± 2.3)

Topical

Solid

0 (0)

0 (0)

0 (0)

0 (0)

0 (0)

Semisolid

10.6 (14)

0 (0)

0 (0)

1.5 (2 ± 0.5)

1.5 (2 ± 1.0)

Liquid

6.0 (8)

0 (0)

0.8 (1 ± 1.0)

2.3 (3 ± 0.8)

3.8 (5 ± 1.1)

Total

60.6 (80)

6.8 (9 ± 1.1)

5.3 (7 ± 1.2)

15.1 (20 ± 1.5)

16.7 (22 ± 1.9)

Grand total

100 (132)

26 (19.7)

16 (12.1)

42 (31.8)

31 (23.5)

  1. Macapa, Brazil, from 2016 to 2017
  2. aMicrobial contamination limits in herbal materials, preparations and finished products according to WHO standards [3]. All experiments were completed in triplicate, (N) represents absolute values as mean and standard deviation