Table 1 Primer sequences and conditions for qRT-PCR
mRNA | Primer sequence | Product size (bp) | Ta (°C) |
|---|---|---|---|
β-actin | F: TCATGAAGTGTGACGTTGACATCC R: GACTGTTACTGAGCTGCGTTTTAC * NM_031144.3 | 311 | 53 |
TNF-α | F: ACTGAACTTCGGGGTGATTG R: GTCGTAGCAAACCACCAAGC * HQ201305.1 | 153 | 56 |
IL-6 | F: ATATGTTCTCAGGGAGATCTTGGAA R: GTGCATCATCGCTGTTCATACA *NM_031512 | 80 | 60 |
GPAT-1 | F: AGACACAGGCAGGGAATCCAC R: AATTCCCGGAGAAGCCCAG *AF021348 | 103 | 56 |
ACC | F: GCCCACTTTCTTCTATCACGCTAA R: GAAGACGGCAGCATGAACTG *XM_017598248.1 | 159 | 56 |
SREBP-1c | F: CAGAGGGACTACAGGCTGAGAAAG R: CACGTAGATCTCTGCCAGTGTTG *NM_001276708.1 | 204 | 56 |