Fig. 9

Effects of BBR on TLR4 and p-AMPK protein levels (a) and the interaction between TLR4 and MyD88 (b) induced by LPS in Raw264.7 cells. N, normal control group; LH, high LPS group; LH-B, high LPS + BBR group. c-e Molecular docking diagrams of the interaction between BBR and TLR4. c 2D docking diagram of the interaction between BBR and TLR4. The black dotted line and green curve indicate a hydrogen bond and hydrophobicity, respectively. BBR can form hydrogen bonds with Thr174, Asn204 and Val203, and can also form hydrophobic interactions with the Asn204 and Gln202 residues in the A chain of TLR4. d The surface structure of the ligand formed around the active centre of the A chain of TLR4. Red, BBR. e The conformation of the interaction between the ligand and the A chain of TLR4 after optimization