Antioxidant and Cytoprotective effects of Pyrola decorata H. Andres and its five phenolic components

Table 1 The IC₅₀ values of LAEP and its five phenolic components in various antioxidant assays

Assays	LAEP (μg/mL)	Protocatechuic acid (μM)	Gallic acid (μM)	Hyperoside (μM)	2′′-O-Galloylhyperin (μM)	Quercetin (μM)	Trolox (μM)
Fe³⁺-reducing	367.6 ± 11.2	105.5 ± 4.1^c	54.3 ± 2.2^a	51.4 ± 1.0^a	48.9 ± 2.5^a	59.3 ± 3.0^b	97.3 ± 9.7
Cu²⁺-reducing	829.0 ± 13.9	183.7 ± 3.7^d	148.3 ± 4.0^c	105.6 ± 4.9^b	71.9 ± 2.7^a	123.5 ± 9.9^b	373.9 ± 17.6
PTIO^•-scavenging (pH 4.5)	131.7 ± 11.9	527.5 ± 6.6^b	181.5 ± 5.6^a	193.7 ± 28.4^a	167.1 ± 14.7^a	185.3 ± 15.3^a	217.7 ± 5.0
PTIO^•-scavenging (pH 7.4)	279.8 ± 58.3	437.0 ± 43.0^d	179.7 ± 10.9^c	135.2 ± 23.3^b	92.2 ± 7.0^a	103.6 ± 4.6^a	142.9 ± 5.0
DPPH^•-scavenging	82.4 ± 5.2	7.6 ± 0.5^c	5.0 ± 0.2^b	3.5 ± 0.1^b	3.0 ± 0.1^a	3.4 ± 0.2^a	17.0 ± 1.5
ABTS^+•-scavenging	42.3 ± 4.3	25.4 ± 0.4^d	11.6 ± 0.4^c	5.9 ± 0.2^b	3.5 ± 0.1^a	3.3 ± 0.1^a	21.8 ± 0.6

The IC₅₀ value was defined as the final concentration corresponding to 50% radical inhibition (or relative reducing power) obtained from the dose-response curves in Additional file 8, as analyzed by Origin 2017 professional software (OriginLab, Northampton, MA, USA). The IC₅₀ values of the five phenolics with different superscripts (^a, ^b, ^c, or ^d) in the same row are significantly different (p < 0.05). Trolox ((±)-6-hydroxyl-2,5,7,8-tetramethlychromane-2-carboxylic acid) served as the positive control. LAEP means the lyophilized aqueous extract of P. decorata. Each experiment was performed in triplicates; the data were recorded as mean ± standard deviation (SD)

ISSN: 2662-7671