In vitro and in silico anti-dengue activity of compounds obtained from Psidium guajava through bioprospecting

Table 1 Cytotoxic concentration 50% (CC₅₀₎, effective concentration 50% (EC₅₀₎, and selectivity index (SI) values of the evaluated ethanolic extracts, fractions, and compounds in VERO cells infected with DENV-2/NG

Type of compound	Scientific name	Family	Voucher number	Plant part	CC₅₀	CE₅₀	SI
Ethanolic extracts	Cymbopogon citratos Staf	Poaceae	JBC 12015	Leaves	155.1	343.1	0.5
	Sarcostemma clausum Jacq	Asclepiadaceae	JBC 2502	Leaves	565.7	458.7	1.2
	Diospyros inconstans Jacq	Ebenaceae	JBC 1438	Bark	727.1	538.6	1.4
	Chrysobalanus icaco L	Chrysobalanaceae	JBC 934	Seeds	550.8	325.8	1.7
	Trichilia hirta L	Meliaceae	JBC 917	Seeds	213.6	62.9	3.4
	Croton malambo Karst	Euphorbiaceae	JBC 12008	Bark	127.3	31.3	4.1
	Ambrosia cumanensis Kunt	Asteraceae	COL 538448	Leaves	112.4	26.7	4.2
	Chenopodium ambrosioides L	Chenopodiaceae	JBC 4005	Leaves	131.6	24.8	5.3
	Cavanillesia platanifolia Bonpl	Bombacaceae	JBC 47576	Almond	252.6	43.5	5.8
	Momordica charantia L	Cucurbitaceae	JBC 793	Seeds	125.3	15.9	7.9
	Mammea americana L	Calophyllaceae	JBC 467	Leaves	440.7	52.3	8.4
	Psidium guajava L	Myrtaceae	JBC 1209	Bark	1000.0	7.8	128.2
Fractions	Psidium guajava L	–	HUA 140931	Pg-YP-I-22A	130.5	134.4	1.0
				Pg-YP-I-22B	308.9	26.5	11.7
				Pg-YP-I-22C	625.7	17.7	35.4
				Pg-YP-I-22D	177.9	56.1	3.2
				Pg-YP-I-22E	102.2	16.7	6.1
Compounds	Pg-YP-I-22C	–	–	Gallic Acid	543.4	25.8	21.1
				Naringin	646.8	47.9	13.5
				Quercetin	659.8	19.2	34.3
				Catechin	833.3	33.7	24.8
				Hesperidin	413.8	225.8	1.8

Group A: No selectivity, SI < 2.0
Group B: Low selectivity, SI ≥ 2.0 and < 5
Group C: Moderate selectivity, SI ≥ 5 and < 10
Group D: High selectivity, SI ≥ 10
• The plants Cymbopogon citratos Staf, Sarcostemma clausum Jacq, Diospyros inconstans Jacq, Chrysobalanus icaco L, Trichilia hirta L and Croton malambo Karst, Chenopodium ambrosioides L, Cavanillesia platanifolia Bonpl, Momordica charantia L, Mammea americana L, Psidium guajava L were identified at the Jardin Botanico de Cartagena (JBC)
• The plant Ambrosia cumanensis Kunt was identified at the Herbarium of the Universidad Nacional de Colombia (COL)
• The plant used to obtain the fractions from Psidium guajava L was identified at the Herbarium of the Universidad de Antioquia (HUA)

ISSN: 2662-7671