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Fig. 4 | BMC Complementary and Alternative Medicine

Fig. 4

From: Puerarin inhibits the osteoclastogenesis by inhibiting RANKL-dependent and –independent autophagic responses

Fig. 4

Under RANKL intervention, BECN1 overexpression reverses Puerarin-inhibited OCP autophagy and osteoclast differentiation. a-b LC3II/I in viruses-transduced OCPs treated with Puerarin (50 μM) along with RANKL for 6 h was detected by Western Blotting. Compared between Group LV-Cont and Group LV-Cont+PR, Group LV-Cont+PR and Groups LV-Atg5/BECN1/Atg7+ PR in the presence of RANKL, respectively, *P < 0.05. c Typical TRAP staining of mature osteoclasts derived from viruses-transduced OCPs under the joint intervention of M-CSF, RANKL and Puerarin for 5 days. Scale bar, 100 μm. d The quantitative results regarding TRAP+ multinucleated cells in C. e-g mRNA expression of MMP-9, TRAP or CTSK in OCPs treated with indicated treatments. Data are presented as mean ± SEM from three independent experiments. Compared between Group LV-Cont and Group LV-Cont+PR, Group LV-Cont+PR and Groups LV-Atg5/BECN1/Atg7+ PR in the presence of RANKL plus M-CSF, respectively, *P < 0.05. ns, no significance; PR, Puerarin

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