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Fig. 2 | BMC Complementary and Alternative Medicine

Fig. 2

From: Puerarin inhibits the osteoclastogenesis by inhibiting RANKL-dependent and –independent autophagic responses

Fig. 2

Overexpression of autophagic gene reverses Puerarin-reduced OCP proliferation. a The proliferation of viruses-transduced OCPs treated with Puerarin (50 μM) for 24 h was detected using EdU kit. Scale bar, 250 μm. b Statistical diagram displayed the percentages of EdU-positive cells in A (50 cells per field, n = 10). c After treatment as described for (a), the OCP proliferation was measured using CCK-8 kit. The group without Puerarin were regarded as the control group (100%). Data are presented as mean ± SEM from three independent experiments. Compared between Group LV-Cont and Group LV-Cont+PR, Group LV-Cont+PR and Groups LV-Atg5/BECN1/Atg7+ PR, respectively, *P < 0.05. PR, Puerarin

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BMC Complementary Medicine and Therapies

ISSN: 2662-7671

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