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Table 2 Method validation for the quantitative determination of four compounds using RP-HPLC

From: Chemical composition, antibacterial activity and related mechanism of valonia and shell from Quercus variabilis Blume (Fagaceae) against Salmonella paratyphi a and Staphylococcus aureus

Peak no. Compounds Retention time Regression equation Precision experiment Repeatability Recovery experiment
Area of peak RSD (%) Area of peak RSD (%) Average recovery rate RSD (%)
1 tannin acid 6.794 Y = 19,106x + 31.263 (R2 = 0.9996) 2492.6 ± 10.1 0.41 159.4 ± 33.44 2.09 105.57 ± 1.75 1.36
2 theophyline 15.605 Y = 30,252x-13.334 (R2 = 0.9995) 3753 ± 5.9 0.20 1346.8 ± 53.74 2.22 102.86 ± 1.25 1.18
3 caffeic acid 27.98 Y = 31,908x + 31.065 (R2 = 0.9994) 4172.2 ± 8.2 0.20 625.26 ± 17.25 2.75 98.36 ± 0.36 0.34
4 ellagic acid 35.447 Y = 206,229x + 1865.8 (R2 = 0.9984) 22,707.5 ± 103.3 0.34 20,841.7 ± 232.01 1.11 102.54 ± 2.83 2.75
  1. Values were expressed in mean ± SD (n = 6).