Isolation of bioactive compounds from Bergenia ciliata (haw.) Sternb rhizome and their antioxidant and anticholinesterase activities

Table 2 Percent DPPH and ABTS radical scavenging potential of crude extract and their sub fractions of Bergenia ciliata rhizome using ascorbic acid as standard

Samples	Concentrations (μg/mL)	DPPH Percent inhibition (mean ± S.E.M)	DPPH IC₅₀ (μg/mL)	ABTS percent inhibition (mean ± S.E.M)	ABTS IC₅₀(μg/mL)
Crude	1000	87.50 ± 0.70*		85.37 ± 2.45 ^ns
	500	77.66 ± 1.20 ^ns		76.33 ± 0.67 ^ns
	250	70.31 ± 0.76 ^ns	62	69.00 ± 1.00 ^ns	70
	125	62.34 ± 2.45 ^ns		57.33 ± 0.66 ^ns
	62.5	50.50 ± 0.70 ^ns		48.45 ± 0.52 ^ns
n-butanol	1000	82.62 ± 0.76***		80.26 ± 1.03***
	500	74.32 ± 0.62**		70.05 ± 0.77***
	250	63.00 ± 1.73***	122	60.30 ± 2.33***	140
	125	47.22 ± 0.66***		44.97 ± 1.09***
	62.5	39.03 ± 0.60***		34.10 ± 0.50***
Ethyl acetate	1000	77.66 ± 2.45***		75.00 ± 0.88***
	500	66.07 ± 0.56***		65.66 ± 0.81***
	250	59.33 ± 1.46***	170	57.02 ± 0.44***	186
	125	44.08 ± 0.73***		42.11 ± 0.66***
	62.5	32.50 ± 3.45***		27.64 ± 2.39***
Chloroform	1000	70.27 ± 0.71***		68.84 ± 0.47***
	500	61.31 ± 0.60***		57.33 ± 0.78***
	250	53.33 ± 1.45***	205	50.22 ± 0.46***	267
	125	40.05 ± 0.56***		35.01 ± 0.52***
	62.5	27.12 ± 0.66***		23.25 ± 0.38***
Aqueous	1000	62.18 ± 0.67***		60.15 ± 0.44***
	500	51.50 ± 0.55***		48.33 ± 1.33***
	250	42.02 ± 0.57***	440	37.64 ± 0.58***	600
	125	28.37 ± 0.65***		24 .62 ± 0.85***
	62.5	16.56 ± 2.38***		13.03 ± 0.86***
n-hexane	1000	54.27 ± 0.72***		52.50 ± 0.50***
	500	42.49 ± 0.50***		40.00 ± 1.08***
	250	34.07 ± 0.58***	830	31.16 ± 0.70***	910
	125	25.31 ± 0.67***		22.06 ± 2.38***
	62.5	12.66 ± 1.45***		10.04 ± 1.54***
Ascorbic acid	1000	92.94 ± 0.86		89.67 ± 0.73
	500	80.29 ± 0.79	50	80.45 ± 0.96	64
	250	71.93 ± 0.45		68.34 ± 2.16
	125	62.90 ± 0.48		58.90 ± 0.85
	62.5	52.88 ± 0.32		49.78 ± 0.76

Ascorbic acid was used as a positive control. Data is represented as mean ± S.E.M; (n = 3). Values significantly different as compared to positive control,*: P < 0.05, ***: P < 0.001, ns: P > 0.05

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