Fig. 3

Apoptotic effects of SN extracts and gemcitabine on PDAC cells. a AsPC1, BxPC3 and SW1990 were treated with 0.1% DMSO, SN extracts, gemcitabine, or combination of both SN extracts and gemcitabine. The cells were then harvested for apoptotic induction using ELISA assay. b Apoptotic cell death in AsPC1, BxPC3 and SW1990 cells was quantified using Annexin V/7-AAD flow cytometry at 72 h following treatment. c The same lysates were tested for TLR-4 expression using ELISA assay. d SW1990 cells were treated with 0.1% DMSO, SN extracts, gemcitabine, or a combination of both SN extracts and gemcitabine, and the proteins were harvested for protein array analysis. e BxPC3 and SW1990 cells were exposed to 0.1% DMSO, SN extracts, gemcitabine, or a combination of both SN extracts and gemcitabine for 48 h. Protein lysates were subjected to SDS-PAGE. GAPDH was used as loading control. Each bar represents the mean ± standard deviation of three independent experiments