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Table 1 Gradient Elution Conditions

From: Baihe Zhimu formula attenuates the efficacy of tamoxifen against breast cancer in mice through modulation of CYP450 enzymes

Type Time (min) A (%) B (%)
HPLC-MS/MS   Acetonitrile 0.1% formic acid solution
0–8 5 95
8–10 10 90
10–20 20 80
20–50 20 80
50–70 55 45
70 100 0
HPLC-UV   Acetonitrile 0.3% formic acid solution + 0.05% Triethylamine solution
0–12 8–13 92–87
12–27 13 87
27–40 13–24 87–76
40–56 24 76
56–57 24–100 76–0
HPLC-ELSD   Acetonitrile 0.3% formic acid solution + 0.05% Triethylamine solution
0–15 24 76
15–20 24–35 76–65
20–30 35 65
30–35 35–100 65–0
35–50 100 0