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Table 1 Gradient Elution Conditions

From: Baihe Zhimu formula attenuates the efficacy of tamoxifen against breast cancer in mice through modulation of CYP450 enzymes

Type

Time (min)

A (%)

B (%)

HPLC-MS/MS

 

Acetonitrile

0.1% formic acid solution

0–8

5

95

8–10

10

90

10–20

20

80

20–50

20

80

50–70

55

45

70

100

0

HPLC-UV

 

Acetonitrile

0.3% formic acid solution + 0.05% Triethylamine solution

0–12

8–13

92–87

12–27

13

87

27–40

13–24

87–76

40–56

24

76

56–57

24–100

76–0

HPLC-ELSD

 

Acetonitrile

0.3% formic acid solution + 0.05% Triethylamine solution

0–15

24

76

15–20

24–35

76–65

20–30

35

65

30–35

35–100

65–0

35–50

100

0