Type | Time (min) | A (%) | B (%) |
---|---|---|---|
HPLC-MS/MS | Â | Acetonitrile | 0.1% formic acid solution |
0–8 | 5 | 95 | |
8–10 | 10 | 90 | |
10–20 | 20 | 80 | |
20–50 | 20 | 80 | |
50–70 | 55 | 45 | |
70 | 100 | 0 | |
HPLC-UV |  | Acetonitrile | 0.3% formic acid solution + 0.05% Triethylamine solution |
0–12 | 8–13 | 92–87 | |
12–27 | 13 | 87 | |
27–40 | 13–24 | 87–76 | |
40–56 | 24 | 76 | |
56–57 | 24–100 | 76–0 | |
HPLC-ELSD |  | Acetonitrile | 0.3% formic acid solution + 0.05% Triethylamine solution |
0–15 | 24 | 76 | |
15–20 | 24–35 | 76–65 | |
20–30 | 35 | 65 | |
30–35 | 35–100 | 65–0 | |
35–50 | 100 | 0 |