Fig. 3

Effect of SEE, and FSEE on induction of apoptotic cell death in HepG2 hepatocellular carcinoma. Cells were treated with SEE, and FSEE for 24 h. a Early and late apoptosis population was analyzed by Annexin V staining assay. b DNA fragmentation was observed by 2% agarose gel electrophoresis. c Nuclear condensation in response to SEE, and FSEE treatment as detected by Hoechst staining assay. Data values were expressed as mean ± SD of triplicate determinations. Significant differences were compared with control at *p < 0.05, **p < 0.01, and ***p < 0.001 using one-way ANOVA