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Table 2 Flow cytometric analysis of cell cycle distribution in HeLa cells treated with GA (in %). The results are presented from 3 independent experiments as mean ± SD; significantly different, * P < 0.05, ** P < 0.01 versus untreated cells (control); + P < 0.05 versus GA. Sub-G0/G1 fraction of cells identified as apoptotic population

From: Oxidative stress mediated by gyrophoric acid from the lichen Umbilicaria hirsuta affected apoptosis and stress/survival pathways in HeLa cells

Treatment Time (h) sub-G0/G1 G0/G1 S G2/M
Control 24 1.50 ± 0.33 57.70 ± 1.54 20.80 ± 1.35 20.00 ± 1.11
48 1.80 ± 0.71 64.40 ± 1.88 17.40 ± 0.89 16.30 ± 0.61
72 2.80 ± 0.25 69.20 ± 1.77 14.50 ± 1.20 13.60 ± 0.55
NAC 24 2.90 ± 1.10 58.90 ± 1.41 18.80 ± 0.99 19.40 ± 1.22
48 3.10 ± 0.77 67.50 ± 2.32 13.80 ± 0.57 15.60 ± 0.88
72 2.70 ± 0.43 70.40 ± 1.19 12.40 ± 0.66 14.50 ± 0.67
GA 24 8.00 ± 1.30* 50.00 ± 2.11* 20.00 ± 1.23 22.00 ± 1.19
48 16.20 ± 1.20** 46.50 ± 2.75** 18.50 ± 1.70 18.90 ± 1.44
72 20.90 ± 1.55** 47.90 ± 1.35** 16.50 ± 1.79 14.80 ± 1.30
NAC/GA 24 4.60 ± 0.21 60.70 ± 1.14+ 18.20 ± 0.73 16.50 ± 1.13
48 7.30 ± 0.76*,+ 60.90 ± 2.10+ 14.30 ± 1.30 17.50 ± 1.90
72 5.50 ± 1.25+ 60.70 ± 1.93*,+ 15.60 ± 0.56 18.20 ± 0.33