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Table 5 The percentage of total glucan, α-glucan, β-glucan, protein and phenolic acid content in mushroom HAEs and ASEs

From: Anti-viral activity of culinary and medicinal mushroom extracts against dengue virus serotype 2: an in-vitro study

Extract Total glucana α-glucanb β-glucanc Proteind Phenolic acide
P. giganteus HAE 27.6 ± 0.2 17.6 ± 0.1 10.1 ± 0.1 18.2 ± 2.3 0.33 ± 0.01
P. giganteus ASE 31.6 ± 0.4 3.5 ± 0.3 28.1 ± 0.5 12.6 ± 0.9 0.18 ± 0.01
L. rhinocerotis HAE 71.8 ± 4 13.5 ± 1.1 58.4 ± 1.1 16.5 ± 2.5 0.32 ± 0.06
L. rhinocerotis ASE 23.4 ± 0 7.8 ± 0.3 15.6 ± 0.3 27.6 ± 0.4 0.25 ± 0.03
S. communae HAE 4.8 ± 0.3 0.2 ± 0 4.6 ± 0.3 18 ± 1.9 0.33 ± 0.06
S. communae ASE 35.8 ± 0.3 1.4 ± 0.1 34.4 ± 0.4 15 ± 0.4 0.31 ± 0.05
H. erinaceus HAE 2.0 ± 0 0.5 ± 0.1 1.6 ± 0.1 20.6 ± 1.7 0.32 ± 0.06
H. erinaceus ASE 2.9 ± 1.7 0.3 ± 0.1 2.7 ± 0.1 12.7 ± 0.8 0.2 ± 0.02
G. lucidium HAE 9.4 ± 1.3 4.5 ± 0.4 4.9 ± 1.8 22 ± 3.7 1.28 ± 0.16
G. lucidium ASE 11.1 ± 0 1.3 ± 0.1 9.8 ± 0.1 19 ± 1.9 0.73 ± 0.08
  1. Percentages of α-glucan, β-glucan, protein, phenolic content in 10 mg/ml mushroom extracts were calculated as the mean ± standard deviation of three independent experiments using the formula listed below:
  2. aTotal glucan: \( \mathrm{Absorbance}\times \frac{100\ \left(\upmu \mathrm{g}\ \mathrm{of}\ \mathrm{the}\ \mathrm{D}\hbox{-} \mathrm{g}\mathrm{lucose}\ \mathrm{standard}\right)}{\mathrm{GOPOD}\ \mathrm{absorbance}\ \mathrm{for}\ 100\;\upmu \mathrm{g}\ \mathrm{of}\ \mathrm{D}\hbox{-} \mathrm{g}\mathrm{lucose}\ \mathrm{standard}}/\mathrm{weight}\ \mathrm{of}\ \mathrm{sample}\times 90 \)
  3. bα-glucan: \( \mathrm{Absorbance}\times \frac{100\ \left(\upmu \mathrm{g}\ \mathrm{of}\ \mathrm{the}\ \mathrm{D}\hbox{-} \mathrm{g}\mathrm{lucose}\ \mathrm{standard}\right)}{\mathrm{GOPOD}\ \mathrm{absorbance}\ \mathrm{for}\ 100\;\upmu \mathrm{g}\ \mathrm{of}\ \mathrm{D}\hbox{-} \mathrm{g}\mathrm{lucose}\ \mathrm{standard}}/\mathrm{weight}\ \mathrm{of}\ \mathrm{sample}\times 9.27 \)
  4. cβ-glucan: Total glucan – α-glucan
  5. dTotal protein content estimated from the bovine serum albumin standard curve (Absorbance at 595 nm = 0.001 (concentration of protein) - 0.166). % protein: concentration of protein (mg)/concentration of extract (10 mg) × 100
  6. eTotal phenolic content estimated from the gallic acid standard curve (Absorbance at 650 nm = 0.939 {concentration of phenolic (mM)} - 0.055). % phenolic: concentration of phenolic (mM) × 170/conc of extract (10 mg) × 100