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Table 1 The primer sequences of GAPDH, NS5 and ENV

From: Anti-viral activity of culinary and medicinal mushroom extracts against dengue virus serotype 2: an in-vitro study

Target gene Sequence Tm
GAPDH-F 5′ GTG GAC CTG ACC TGC CGT CT 3′ 58.4
GAPDH-R 5′ GGA GGA GTG GGT GTC GCT GT 3′ 58.4
ENV-F 5′ ACA AGT CGA ACA ACC TGG TCC AT 3′ 58.3
ENV-R 5′ GCC GCA CCA TTG GTC TTC TC 3′ 59.0
NS5-F 5′ GGA AGG AGA AGG ACT GCA CA-3′ 53.8
NS5-R 5′ ATT CTT GTG TCC CAT CCT GCT 3′ 54.7
  1. The nucleotide sequences for primers were obtained from Poh et al. [14] and Kong et al. [15]