Fig. 2
From: Berberine inhibits NLRP3 Inflammasome pathway in human triple-negative breast cancer MDA-MB-231 cell
Effect of BBR on colony formation potential of MDA-MB-231 cells. Cells were seeded in 6-well plates at 1000 cells per well following treatment with indicated concentrations of BBR for 24 h. The medium was then replaced with fresh media, and the cells were allowed to grow for 12 days at 37 °C in a humidified atmosphere (5% CO2 in air) before staining with Giemsa stain solution. a Typical results of the clonogenic assay. b The surviving fraction of treated MDA-MB-231 cell line is presented as mean ± S.D. (n = 3) of three independent experiments. * p < 0.05, ** p < 0.01, compared to the control