Fig. 3

cell cycle arrest effect of Huaier and 5-FU in single agent or combination on Huh28 cells. a DNA content-based cell cycle of Huh28 cells after 48 h Huaier and 5-FU alone or in combination treatment were analyzed using Flow cytometry. b Percentage of cells in S phases. c, d and e The expression variations of cell cycle related proteins under different treatment conditions were detected using Western blot, and relative optical densities of proteins were analyzed by Image J. All results are expressed as mean ± SD of three independent experiments. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, compared with control group, while ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, compared with combination group