Fig. 9

Effect of CR on the expression of NFATc1, c-Fos and CTK in OVX-induced femur. a Immunohistochemistry staining of NFATc1, c-Fos and CTK was conducted. One representative femur from each group was captured using a light microscope. The red arrowheads indicate each indicators-positive cells. (100X and 200X magnification, Scale bars are 200 and 100 μm, respectively). b Quantification of NFATc1-positive cells, c c-Fos-positive cells and d CTK-positive cells in femur were examined. Data represent the mean ± S. E of each experimental groups. ^##p < 0.01 compared with the Sham and ^**p < 0.01, *p < 0.05 compared with OVX