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Fig. 6 | BMC Complementary and Alternative Medicine

Fig. 6

From: Ethanol extract of asiasari radix preferentially induces apoptosis in G361 human melanoma cells by differential regulation of p53

Fig. 6

cDNA microarray-based identification of MDM2 and CFLAR as genes responsible for ARE-mediated apoptosis in G361 cells. Genes showing more than 2-fold change after ARE treatment identified by cDNA microarray analysis using total RNA from G361 and HaCaT cells were classified into two groups; (a) apoptosis-related genes and (b) ROS-related genes. The change in gene expression levels in each group after ARE treatment is expressed as a heat map. The color intensity represents the extent of change; green indicates a decrease in gene expression, whereas red indicates an increase in gene expression. c The results of RT-PCR analysis of MDM2 and CFLAR genes are shown. GAPDH was used as a loading control. The relative expression of MDM2 and CFLAR mRNA were represented as graphs (bottom panel). Data represent the mean ± SEM (n = 3). The significant changes after ARE treatment were marked as * (p < 0.05) Table 1. The list of ROS and apoptosis related genes those differently regulated by ARE in G361 and HaCaT cells. The significant changes after ARE treatment were marked as * (p < 0.05)

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