Fig. 3

Migratory effects of Fraction 2 on keratinocytes in the in vitro scratch wound assay. a Migration of HaCaT cells observed at ×100 magnification, 0 and 24 h post-scratching. b Rate of wound healing, as calculated using Image J software. Wound closure rate % = (the scarification area at 0 h – the scarification area at 24 h) / the scarification area at 0 h × 100%. Three randomly captured scarification areas were measured in each well. Results are expressed as the mean ± SEM of at least three determinations for each test from three independent experiments. Statistical significance of differences: *, p < 0.05; **, p < 0.01; ***, p < 0.001 (* comparisons versus control conditions)