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Fig. 6 | BMC Complementary and Alternative Medicine

Fig. 6

From: Synthesized natural peptides from amphibian skin secretions increase the efficacy of a therapeutic vaccine by recruiting more T cells to the tumour site

Fig. 6

Caerin 1.1 and 1.9 attract more T cells to the tumour site Four groups of five C57/BL6 mice were subcutaneously transplanted with 2 × 105 of TC-1 tumour cells. Four days after tumour inoculation, two groups were immunised twice 7 days apart with Ex/MPLA/anti-IL-10 receptor antibodies as described above. One day after the primary immunisation, mice were either injected for 7 consecutive days with 30 μg of caerin 1.1 and caerin 1.9, or PBS. When the tumor diameter was 1 cm in the control group, the mice were sacrificed and the tumor tissues were dissociated, digested and analysed by flow cytometry for surface molecules expression of CD45.2, CD4, CD8a and NK1.1. (a) Percentage of CD45.2 cells; (b) Percentage of CD3 + CD8+ cells gated on CD45.2 cells; (c) Percentage of CD3 + CD4+ cells gated on CD45.2 cells; (d) Percentage of CD3-NK1.1+ of CD45.2 cells in tumor tissue.

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